鲍晓明
发布时间: 2017-10-30 浏览次数: 1426

  

姓 名

鲍晓明

性 别

出生年月

1961.11

学 历

博士

毕业时间

1999.06

专 业

微生物学

电 话

18678789961

邮 编

250353

E-Mail

baoxm@qlu.edu.cn

单 位

生物工程学院

通信地址

济南市长清区大学路3501号齐鲁工业大学食工楼A503

教育背景及工作简历

1979.9-1983.7,山东大学生物系微生物专业,理学学士;

1983.7-1985.9,华中农业大学土化系农业微生物教研室,助教;

1985.9-1988.7,山东农业大学植保系真菌生物学专业,农学硕士;

1988.7-1993.9,山东大学生命学院,微生物技术国家重点实验室,助教;

1993.9-1996.12,山东大学生命学院,微生物技术国家重点实验室,讲师;

1993.9-1997.7,山东大学生命科学院,微生物技术国家点实验室,理学博士(在职攻读)

1995.1-1996.1,瑞典隆德大学应用微生物系,联合培养博士生合作研究;

2000.1-2000.12,瑞典歌德堡大学细胞分子生物学及微生物学系,酵母研究中心,博士后;

2002.9-2017.12,山东大学生命科学院,微生物技术国家点实验室,教授、博导;

2007.7-2007.9,日本山口大学酵母研究组,友好学校交流高级访问学者;

2017.12年起,于齐鲁工业大学生物工程学院,教授,山东省微生物工程重点实验室副主任。

研究方向

研究方向为微生物遗传学与分子生物学。主要关注酿酒酵母(Saccharomyces cerevisiae)开展代谢工程、系统生物学、合成生物学的研究。根据代谢途径工程原理,对酿酒酵母代谢途径进行理性设计并辅助非理性研究措施,开展拓宽乙醇发酵底物范围酵母代谢工程研究,使酿酒酵母获得利用木糖产生乙醇的能力,拓宽并提高乙醇发酵的底物利用,为实现木质纤维素水解液全糖发酵乙醇产业化技术奠定了扎实的基础。相关结果始终国际同类研究的前沿水平同步。同时在各类组学研究的基础上,揭示酿酒酵母高效共转化木糖/葡萄糖,及高抗逆性的分子机理。并开展酿酒酵母高效分泌元件与异源蛋白兼容性的规模化研究,开展酿酒酵母糖运输蛋白的研究。今年来对生物基化学品的转化研究特色为理论联系实际,在酿酒酵母分子生物学、遗传学的基础与应用技术领域已形成明显研究特色。

研究课题(按时间顺序)

  1. 主持,山东省重点研发计划:2017CXGC1105,甘薯生物质制备新型食品增味剂关键技术研发及产业化,200万元。

  2. 参加,山东省科技重大专项(新兴产业)2015ZDXX0403B02,秸秆制浆废渣酶解发酵生产燃料乙醇技术,300万元。

  3. 主持,国家自然科学基金面上项目:31470219,酿酒酵母高效分泌元件与异源蛋白兼容性的规模化研究,85万元。

  4. 主持,山东省秸秆生物炼制技术重点实验室项目:五碳糖和六碳糖共发酵产乙醇和大宗化学品高效工业菌株选育和发酵条件优化,15

  5. 元。

  6. 支持国家能源局子项目:NY20130402,万吨级纤维素乙醇生产关键技术研究及示范,145万元。

  7. 主持,车用生物燃料技术国家重点实验室开放课题项目:酿酒酵母对木质纤维素预处理毒性产物代谢生理的响应及其抗逆性机理研究,8万元。

  8. 主持,863项目课题“工业酵母菌种的基因组全局扰动/2012AA022106”任务:戊糖己糖共发酵高产乙醇菌株开发,82万元。

  9. 主持,丹麦诺维信公司合作研发项目:C5/C6共发酵菌株乙醇发酵产业化研究,150万元。

  10. 主持,国家自然科学基金面上项目:31270151,木糖异构酶在酿酒酵母中高效转化木糖的适配性分子机制研究,82万元。

  11. 参加,973子课题:2011CB707405,纤维降解组分高效生物转化的代谢网络调控与改造, 86 万元。

  12. 主持,国家自然科学基金面上项目:批准号31070096,酿酒酵母木质纤维素全糖乙醇发酵代谢工程基础问题的系统生物学研究,34万元。

  13. 主持,国家科技部国际科技合作项目,2010DFA32560,第二代燃料乙醇生产集成技术研究,83万元。

  14. 主持,马来西亚棕榈油协会合作研发项目:棕榈生物质乙醇转化研究,40万元。

  15. 主持,荷兰帝斯曼公司合作研发项目:在酿酒酵母表面展示木糖异构酶,40万元。

  16. 主持,意大利康泰斯公司合作研发项目:木质纤维素乙醇生产关键技术研究,65万元。

  17. 主持,英国壳牌公司合作研发项目:纤维素酶基因在酿酒酵母中的表达,20万元。

  18. 主持,国家科技部863计划项目:2007AA05Z402:生物质全糖乙醇发酵酿酒酵母工程菌的构建及发酵技术研究,65万元。

  19. 主持,国家自然科学基金国际合作项目:批准号30740420552,酿酒酵母Bdf1p 转录因子在盐胁迫应答反应调控机制的研究,25万元。

  20. 参加,973子课题:2007CB7070803,代谢途径的分子调控和功能效应,20万元。

  21. 主持,国家自然科学基金面上项目:30671143,酿酒酵母盐胁迫应答反应表观遗传调控机制的研究,26万元。

  22. 主持,国家自然科学基金面上项目:30570031, 酿酒酵母含Bromodomain转录因子Bdf1p在高盐胁迫反应中调控机制的研究,26万元。

  23. 主持,国家自然科学基金面上项目:50273019, 酵母工程菌高效转化生物质资源生产燃料乙醇的研究及应用,23万。

  24. 主持,教育部留学回国人员科研资助项目:转座标记技术在酿酒酵母抗渗功能基因研究中应用。

  25. 主持,国家自然科学基金面上项目:30170021,建立转座标记技术大规模探寻酵母抗渗基因及其功能的研究,16万元。

  26. 参加,国家自然科学基金面上项目:39770012,对瑞氏木霉基因定位置换整合构建木糖醇生产菌,16万元。

  27. 主持,山东省自然科学基金:Q97DO1133,克隆细菌木糖异构酶基因构建木糖发酵酿酒酵母及发酵产物分析。

  28. 主持,教育部留学回国人员科研资助项目:酿酒酵母木糖代谢工程菌的构建及其乙醇发酵的研究。

  29. 主持,高等学校博士学科点专项科研基金:20040422056,高效稳定杀虫白僵菌工程菌的构建及其应用研究。

部分论文:

    1. Chengqiang Wang, Yanwei Li1, Chenxi Qiu, Shihao Wang, Jinjin Ma, Yu Shen, Qingzhu Zhang, Binghai Du, Yanqin Ding, Xiaoming Bao*. Identification of important amino acids in Gal2p for improving the L-arabinose transport and metabolism in Saccharomyces cerevisiae. Frontiers in Microbiology, 2017, 8: 1391.

    2. Chengqiang Wang, Jianzhi Zhang, Chenxi Qiu, Shihao Wang, Yu Shen, Binghai Du, Yanqin Ding*, Xiaoming Bao*. Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering. BioMed Research International, 2017, 2017: 5318232.

    3. Liu, Gang, Zhang, Qiang, Li, Hongxing, Qureshi, Abdul Sattar, Zhang, Jian, Bao, Bao Xiaoming*, Jie. Dry biorefining maximizes the potentials of simultaneous saccharification and co-fermentation for cellulosic ethanol production. Biotechnology and Bioengineering. 2017; 1–10. https://doi.org/10.1002/bit.26444

    4. Wang, X. N. and X. M. Bao*. "The absence of the transcription factor Yrr1p, identified from comparative genome profiling, increased vanillin tolerance due to enhancements of ABC transporters expressing, rRNA processing and ribosome biogenesis in Saccharomyces cerevisiae" Frontiers in microbiology 2017, DOI: 10.3389/fmicb.2017.00367.  

    5. Xiaoxu Chen, Xiaoyu Yang, Yu Shen, Jin Hou, and Xiaoming Bao, Increasing Malonyl-CoA Derived Product through Controlling the Transcription Regulators of Phospholipid Synthesis in Saccharomyces cerevisiae, ACS Synth. Biol. 2017, DOI: 10.1021/acssynbio.6b00346

    6. Hongting Tang, Meihui Song, Yao He, Jiajing Wang, Shenghuan Wang, Yu Shen, Jin Hou*and Xiaoming Bao1,2 Engineering vesicle trafcking improves the extracellular activity and surface display effciency of cellulases in Saccharomyces cerevisiae. Biotechnology for Biofuels. DOI 10.1186/s13068-017-0738-8

    7. Zhao J, Li C, Zhang Y, Shen Y, Hou J, Bao X*. Dynamic control of ERG20 expression combined with minimized endogenous downstream metabolism contributes to the improvement of geraniol production in Saccharomyces cerevisiae. Microb Cell Fact. 2017 Jan 31;16(1):17. doi: 10.1186/s12934-017-0641-9.

    8. Chen, L., Wang, M., Hou, J., Fu, J., Shen, Y., Liu, F., & Bao*, X., HAL2 overexpression induces iron acquisition in bdf1Δ cells and enhances their salt resistance. Current Genetics, 2016, 63(2), 229-239, DOI 10.1007/s00294-016 -0628-9.

    9. Wang X, Liang Z, Hou J, Bao X*, Shen Y: Identification and functional evaluation of the reductases and dehydrogenases from Saccharomyces cerevisiae involved in vanillin resistance. BMC Biotechnology 2016, 16(1):1-9.

    10. Jianzhi Zhao#, Xiaoming Bao#, Chen Li, Yu Shen, Jin Hou. Improving monoterpene geraniol production through geranyl diphosphate synthesis regulation in Saccharomyces cerevisiae, Appl Microbiol Biotechnol. 2016 May;100(10): 4561-71, DOI 10.1007/s00253-016-7375-1

    11. Hongting Tang, Shenghuan Wang, Jiajing Wang, Meihui Song, Mengyang Xu, Mengying Zhang, Yu Shen, Jin Hou & Xiaoming Bao*, N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae. Scientific RepoRts | 6:25654 | DOI: 10.1038/srep25654.

    12. Li, H., Shen, Y., Wu, M., Hou, J., Jiao, C., Li, Z., Liu, X., Bao, X*. 2016. Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production. Bioresources and Bioprocessing, 3(1), 51.

    13. Hou J, Jiao C, Peng B, Shen Y, Bao X,” Mutation of a regulator Ask10 pimproves xylose isomerase activity through up-regulation of molecular chaperones in Saccharomyces cerevisiae” Metabolic Engineering, 2016, (38):241-250

    14. Jin Hou, Yu Shen, Chunlei Jiao, Xingjing Zhang, Xiaoming Bao*, Characterization and evolution of xylose isomerase screened from the bovine rumen metagenome in Saccharomyces cerevisiae. J Biosci Bioeng. 2016, Jul 6, 121(2): 160-165.

    15. C. Wang#, X. Bao#, Y. Li, C. Jiao, J. Hou, Q. Zhang, W. Zhang, W. Liu, Y. Shen, Cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization, Metab. Eng. 30 (2015) 79–88.

    16. Wang, C #., Bao, X #., Li, Y., Jiao, C., Hou, J., Zhang, Q., Zhang, W., Liu, W., Shen, Y. *  Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization. Data Brief, 2015. 4, 119-26.  

    17. Tang H#, Bao X#, Shen Y, Song M, Wang S, Wang C, Hou J: Engineering protein folding and translocation improves heterologous protein secretion in Saccharomyces cerevisiae. Biotechnology and Bioengineering, 2015, 112(9):1872-82

    18. Fang, Z., Liu, X., Chen, L., Shen, Y., Zhang, X., Fang, W., Wang, X., Bao, X*., Xiao, Y*. Identification of a laccase Glac15 from Ganoderma lucidum 77002 and its application in bioethanol production. Biotechnol Biofuels, 20158: 54.

    19. Li, H., Wu, M., Xu, L., Hou, J., Guo, T., Bao, X., Shen, Y*. Evaluation of industrial Saccharomyces cerevisiae strains as the chassis cell for second-generation bioethanol production. Microbial Biotechnology2015 8(2), 266-74.

    20. Fu, J., Hou, J., Chen, L., Wang, M., Shen, Y., Zhang, Z., Bao, X*. , The Yeast BDF1 Regulates Endocytosis via LSP1 Under Salt Stress. Curr Microbiol. 2015 May; 70(5):671-8.

    21. Yu Shen, Hongxing Li, Xinning Wang, Xiaoran Zhang, Jin Hou, Linfeng Wang, Nan Gao, Xiaoming Bao*. The high vanillin tolerance of an evolved Saccharomyces cerevisiae industry strain due to its enhanced vanillin reduction and antioxidantive capacity. Journal of Industrial Microbiology & Biotechnology, 2014, 41, 11, 1637-1645

    22. Lili Xu, Yu Shen, Jin Hou, Hongting Tang, Chengqiang Wang, Xiaoming Bao*, Promotion of Extracellular Activity of Cellobiohydrolase I from Trichoderma reesei by Protein Glycosylation Engineering in Saccharomyces cerevisiae, Current Synthetic and Systems Biology 2014, 2:2

    23. Lei Chen, Mingpeng Wang, Jin Hou, Liangyu Liu, Jiafang Fu, Yu Shen, Zhaojie Zhang, Xiaoming Bao*, Regulation of Saccharomyces cerevisiae MEF1 by Hda1p affects salt resistance of bdf1Δ mutant, FEMS Yeast Res (2014) 14(4): 575-85 .

    24. Lili Xu, Yu Shen, Jin Hou, Bingyin Peng, Hongting Tang, Xiaoming Bao*Secretory pathway engineering enhances secretion of cellobiohydrolase I from Trichoderma reesei in Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 2014,117(1), 45-52

    25. Jin Hou, Fan Suo, Yu Shen, Xiaoming Bao*Fine-tuning of NADH Oxidase Decreases Byproduct Accumulation in Respiration Deficient Xylose Metabolic Saccharomyces cerevisiae, BMC Biotechnology 2014, 14,13

    26. Weixin Zhang, Yanbo Kou, Jintao Xu, Yanli Cao, Guolei Zhao, Jing Shao, Hai Wang, Zhixing Wang, Xiaoming Bao, Guanjun Chen, Weifeng Liu, Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis, Journal of Biological Chemistry , 2013, 288(46):32861-72

    27. Chengqiang Wang, Yu Shen, Yanyan Zhang, Fan Suo, Jin Hou, Xiaoming Bao*, Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport in Saccharomyces cerevisiae. Biomed Res Int. 2013:461204, 1-9.

    28. Hongting Tang, Jin Hou, Yu Shen, Lili Xu, Hui Yang, Xu Fang, Xiaoming Bao*High β-glucosidase secretion in Saccharomyces cerevisiae improves the efficiency of cellulase hydrolysis and ethanol production in simultaneous saccharification and fermentation, Journal of Microbiology and Biotechnology, 2013, 23(11):1577-85

    29. Chengqiang Wang, Yu Shen, Jin Hou, Fan Suo, Xiaoming Bao*, An assay for functional xylose transporters in Saccharomyces cerevisiaeAnalytical biochemistry, 2013, 442 (2013) 241- 248

    30. Shen Y, Hou J, Xiaoming Bao*. Enhanced xylose fermentation capacity related to an altered glucose sensing and repression network in a recombinant Saccharomyces cerevisiae. Bioengineered. 2013 Jun 26: 4(6), 1-3.

    31. Lei Chen, Liangyu Liu, Mingpeng Wang, Jiafang Fu, Zhaojie Zhang, Jin Hou , Xiaoming Bao*. Hal2p functions in Bdf1p-involved Salt Stress Response in Saccharomyces cerevisiae. PLOS ONE. 2013 Apr 17;8(4):e62110.

    32. Jiafang Fu, Jin Hou, Liangyu Liu, Lei Chen, Mingpeng Wang,Yu Shen, Zhaojie Zhang, Xiaoming Bao*. Interplay between BDF1 and BDF2 and their roles in regulating the yeast salt stress response. FEBS J. 2013, 280: 1991-2001.

    33. Yu Shen; Xiao Chen; Bingyin Peng; Liyuan Chen; Jin Hou; Xiaoming Bao*, An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained through adaptive evolution and its global transcription profile, Applied Microbiology and Biotechnology, 2012, 96:1079–1091

    34. Bingyin Peng, Yu Shen, Xiaowei Li, Xiao Chen, Jin Hou, Xiaoming Bao*, Improvement of xylose fermentation in respiratory-deficient xylose-fermenting Saccharomyces cerevisiae, Metabolic Engineering, 2012, 14: 9-18

    35. Qiuqiang Gao, Qun Ren, Liang-Chun Liou, Xiaoming Bao*, Zhaojie Zhang,  Mitochondrial DNA protects against salt stress-induced cytochrome c-mediated apoptosis in yeastFEBS Letters, 2011 585(15):2507-2512

    36. Lei Ji, Yu Shen, Lili Xu, Bingyin Peng, Yazhong Xiao, Xiaoming Bao* Enhanced resistance of Saccharomyces cerevisiae to vanillin by expression of lacA from Trametes sp. AH28-2, Bioresource Technology 2011, 102, 8105–8109

    37. 彭炳银,陈晓,沈煜*,鲍晓明,不同启动子控制下木酮糖激酶的差异表达及其对酿酒酵母木糖代谢的影响,微生物学报(Acta Microbiologica Sinica),201151, 914-922

    38. 徐丽丽, 沈煜,鲍晓明*,酿酒酵母纤维素乙醇统合加工(CBP)的策略及研究进展,生物工程学报,2010, 25; 26(7): 1–10

    39. Xiaoran Zhang, Yu Shen, Wenlong Shi, Xiaoming Bao*, Ethanolic Cofermentation with Glucose and Xylose by the Recombinant Industrial Strain Saccharomyces cerevisiae NAN-127 and the Effect of Furfural on Xylitol Production, Bioresource Technology, 2010, 101(18): 7104–7110

    40. Huaiwei Liu, Bo Zhang, Changsong Li, Xiaoming Bao*, Knockdown of chitosanase expression in the phytopathogenic fungus Fusarium solani and its effect on pathogenicity, Curr Genet201056:275–281

    41. Xu Fang, Yu Shen, Jian Zhao, Xiaoming Bao, Yinbo Qu *. Status and Prospect of Lignocellulosic Bioethanol Production in China. Bioresource Technology2010, 4814–4819

    42. 刘怀伟, 鲍晓明*,腐皮镰孢菌壳聚糖酶的酶学性质研究及其在酿酒酵母工业菌株中的表达,微生物学报,2009,49(12):1607-1612

    43. 李洪兴,张笑然,沈煜,鲍晓明,纤维素乙醇生物加工过程中的抑制物对酿酒酵母影响及应对措施,生物工程学报,2009,25(9)1321-1328

    44. Jin Hou, Goutham N. Vemuri, Xiaoming Bao, Lisbeth Olsson, Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae, Appl Microbiol Biotechnol, 200982:909-919

    45. Huaiwei Liu, Xiaoming Bao*, Overexpression of the chitosanase gene in Fusarium solani via Agrobacterium tumefaciens-mediated transformation, Current Microbiology, 2009, 58(3):279-282.

    46. Xiangyong Liu , Hui Yang, Xiaohua Zhang, Liangyu Liu, Ming Lei, Zhaojie Zhang Xiaoming Bao*, Bdf1p Deletion Affects Mitochondrial Function and Causes Apoptotic Cell Death under Salt Stress, FEMS Yeast Research, 2009 9:240-246

    47. Yu Shen, Yan Zhang, Tao Ma, Xiaoming Bao*, Fengguang Du, Guoqiang Zhuang, Yinbo QuSimultaneous saccharification and fermentation of acid-pretreated corncobs with a recombinant Saccharomyces cerevisiae expressing β-glucosidaseBioresource Technology2008, 99: 5099–5103

    48. J. Hou, Y. Shen, X.P. Li and Xiaoming Bao*, Effect of the reversal of coenzyme specificity by expression of mutated Pichia stipitis xylitol dehydrogenase in recombinant Saccharomyces cerevisiae, Letters in Applied Microbiology, 2007, 45184-189

    49. Xiangyong Liu, Xiaohua Zhang, Chao Wang, Liangyu Liu, Ming Lei, Xiaoming BaoGenetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae, Current Microbiology, 2007, 54325–330

    50. 沈煜,郑华军,王颖,鲍晓明*,曲音波,白凤武,木酮糖激酶表达水平对酿酒酵母木糖代谢产物流向的影响,生物化学与生物物理进展(SCI),200431(8)746-751

    51. Ying Wang, Wen-Long Shi, Xiang-Yong Liu, Yu Shen, Xiao-Ming Bao*, Feng-Wu Bai & Yin-Bo QuEstablishment of a xylose metabolic pathway in an industrial strain of Saccharomyces cerevisiaeBiotechnology Letters200426(11): 885-890

授权专利:

  1. 一株持续高效分泌β-葡萄糖苷酶的重组酿酒酵母重组菌株及其应用,ZL201310187648.X

  2. 一株能够代谢木糖的酿酒酵母菌株,ZL201210088133.X

  3. 一株分泌表达瑞氏木霉外切葡聚糖酶I的重组酿酒酵母菌株及应用,ZL201210044698.8

  4. 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株,ZL201210044319.5

  5. 一种木糖转运能力高的酿酒酵母菌株及其应用,ZL201210007431.1

  6. 酿酒酵母菌株及筛选表达有活性的木糖转运蛋白的酿酒酵母菌株的方法,ZL201110420908.4

  7. Nucleic acid molecule encoding xylose isomerase and xylose isomerase encoded by the nucleic acid molecule,国际专利PCT (2011/001014),美国:US 8,586,336 B2欧盟:EP 2679686

  8. 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶,ZL 201110042170.2。  

  9. 一株耐受香草醛的酿酒酵母,ZL201010251041.X

  10. 一种重组酿酒酵母及其在生产木糖醇中的应用,ZL200810138564.6

  11. 一种木糖异构酶及其编码基因与应用,ZL200810138563.1

  12. 一种利用葡萄糖木糖共发酵生产酒精的方法,ZL200610070207.1

  13. 一株产壳聚糖酶的腐皮镰刀菌突变株及其在制备壳寡糖中的应用,ZL 200610045294.5

【奖励和荣誉】

2016年,获山东大学“优秀党员”荣誉称号。

2013年,获中国专利优秀奖。“利用玉米芯加工残渣发酵生产纤维素酒精的方法”

2011年,获山东大学第三届“我心目中的好导师”荣誉称号

2010年,获山东省科学进步一等奖(第六位)。“木糖渣生产纤维乙醇”

1999年,获山东省优秀博士学位论文奖(证书编号1999B017)。“克隆不同木糖代谢基因酿酒酵母工程菌株的构建及产物分析”

1993年,获山东省教学成果二等奖。

【学术兼职】

中国微生物学会普及教育工作委员会,委员。

山东省遗传学会,理事。

山东省分子与生化学会,理事。


        



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