本硕授课课程: 讲授本科生课程: 《生物工程设备》、《生物制药设备(GMP)》、《生物工程设备课程设计》 讲授研究生课程: 《现代工业发酵调控学》、《发酵过程优化与控制》 纵向课题: 1、基于分子水平调控枯草芽孢杆菌芽孢表面高效稳定展示海藻糖合酶体系研究,31501413,国家自然科学基金青年基金项目,25.2万元,主持; 2、一步混菌发酵制备维生素C,2022CXGC010506,山东省重点研发计划(重大科技创新工程)项目,1000万元,主持; 3、结晶山梨醇绿色制备关键技术研发,2018年度山东省重点扶持区域引进急需紧缺人才项目,75万元,主持; 4、枯草杆菌芽孢表面展示葡萄糖氧化酶关键技术及酶电极研制,2017GSF221019,山东省重点研发计划(公益类),15万元,主持; 5、枯草芽孢表面展示GOD生物酶电极关键技术,2017BSHZ021,齐鲁工业大学青年博士合作基金,10万元,主持; 6、柠檬酸、葡萄糖酸的生物转化技术(子课题),SQ2010AA0222655001,国家“863”计划,208万元,参与; 7、多主枝孢霉QYW522合成10-HDA的代谢途径及关建酶研究,31171727,国家自然基金面上项目,64万元,参与; 8、L-赖氨酸高效生产关键技术与产业化示范,2019JZZY011003,山东省重点研发计划(重大科技创新工程)项目,970万元,参与; 9、淀粉基稀有功能糖及糖醇生物合成细胞工厂构建与产业化示范,2020CXGC010603,山东省重点研发计划(重大科技创新工程),1071万元,参与; 10、生物合成10-HDA热带假丝酵母工程菌的构建与代谢控制,ZR2017ZB0208,山东省自然科学基金重大基础研究项目,90万元,参与; 11、炎性肠病医用食品原料的生物炼制关键技术及其全营养配方食品的研究与应用,2018YYSP002,山东省重点研发计划(医用食品专项计划),100万元,参与; 12、高倍表达海藻糖合酶工程菌构建与应用研究,2011GSF12101,山东省科技发展计划,40万元,参与; 横向课题: 微生物发酵饲料制备关键技术开发,150万元,主持; 新型发酵产品生产技术开发及产业化示范,150万元,主持; 微生物筛选、发酵及其制剂工艺开发,108万元,主持; 新型生物发酵产品的制备技术开发与示范,100万元,主持; C6H13NO5和(C14H20NO11Na)n项目,100万元,主持; 疏水性纤维素醚生产技术及产品开发,60万元,主持; 产甲醇微生物菌系及其制备装置开发,50万元,主持。 主要论文: 1. Hongling Liu, Zhicheng Xue, Feng Liang, BoBo Miao, Tengfei Wang*, Di Huang*, Haibo Yuan, Yi Jiang. Enhancement of the wheat straw pretreatment in pulping:The microbial intelligent response. Industrial Crops and Products, 2025, 223:120109. IF:5.6, (5/8)通讯作者,一区(第一单位) 2. Qian Liu#, Meng Liu#, Wenhu Chen, Haibo Yuan, Yi Jiang, Di Huang, Hongling Liu* and Tengfei Wang*. Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects. Journal of Agricultural and Food Chemistry, 2024, 72(3):1419-1428. IF:5.7, (8/8)通讯作者,一区(第一单位) 3. Kaiquan Liu#, Shan Wei#, Longxiang Song, Hongling Liu* and Tengfei Wang*. Conductive Hydrogels-A Novel Material: Recent Advances and Future Perspectives. Journal of Agricultural and Food Chemistry, 2020, 68(28):7269-7280. IF:5.7, (5/5)通讯作者,一区(第一单位) 4. Hongling Liu#, Song Wang#, Longxiang Song, Haibo Yuan, Kaiquan Liu, Wu Meng* and Tengfei Wang*. Trehalose Production Using Recombinant Trehalose Synthase in Bacillus subtilis by Integrating Fermentation and Biocatalysis. Journal of Agricultural and Food Chemistry, 2019, 67(33):9314-9324. IF:5.7, (7/7)通讯作者,一区(第一单位) 5. Tengfei Wang*, Di Huang*, Xiao Wang, Dejun Chang, Hongling Liu, Cheng Zhong, Zhaoliang Wu. Microbubble-foam fractionation enabled long-term caffeic acid stability in the separation process: Self-assembly WSP as collector. Journal of Molecular Liquids, 2021, 342:117467, IF:5.3, (1/7)通讯作者,一区(第一单位) 6. Shan Wei, Kaiquan Liu*, Xingxiang Ji, Tengfei Wang*, Ruiming Wang*. Application of enzyme technology in biopulping and biobleaching. Cellulose, 2021, 28(16):10099-10116. IF:4.9, (4/5)通讯作者,一区(第一单位) 7. Hongling Liu#, Shaojie Yang#, Xihui Wang# and Tengfei Wang*. Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis spores. Microbial Cell Factories, 2019, 18:100. IF:4.3, (4/4)通讯作者,一区(第一单位) 8. Bobo Miao, Di Huang*,Tengfei Wang*, Hongling Liu, Zhifeng Hao, Haibo Yuan, and Yi Jiang. Enhancing trehalose production via Bacillus species G1 cyclodextrin glucanotransferase mutants: modifying disproportionation characteristics and thermal stability. Frontiers in Microbiology, 2024, 15:1500232. IF:4, (3/7)通讯作者,二区(第一单位) 9. Wentao Yao, Kaiquan Liu*, Hongling Liu, Yi Jiang, Ruiming Wang, Wei Wang, and Tengfei Wang*. A Valuable Product of Microbial Cell Factories: Microbial Lipase. Frontiers in Microbiology, 2021, 12:743377. IF:4, (7/7)通讯作者,二区(第一单位) 10. Wu Meng, Feng Ding, Ruiming Wang* andTengfei Wang*. Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation. Scientific Reports, 2020, 10(1):3544. IF:3.8, (4/4)通讯作者,二区(第一单位) 11. Hongling Liu, Hao Liu, Shaojie Yang, Ruiming Wang,Tengfei Wang*. Improved Expression and Optimization of Trehalose Synthase by Regulation of P-glv in Bacillus subtilis. Scientific Reports, 2019, 9:6585. IF:3.8, (5/5)通讯作者,二区(第一单位) 12. Xiangyi Wang#, Yi Jiang#, Hongling Liu, Haibo Yuan, Di Huang and Tengfei Wang*. Research progress of multi-enzyme complexes based on the design of scaffold protein. Bioresources and Bioprocessing, 2023, 10(1):72. IF:4.3, (6/6)通讯作者,三区(第一单位) 13. Xiangyi Wang, Yi Jiang, Hongling Liu, Xinyi Zhang,Haibo Yuan, Di Huang and Tengfei Wang*. In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein. Frontiers in Bioengineering and Biotechnolog, 2023, 11:1251298. IF:4.3, (7/7)通讯作者,三区(第一单位) 14. Haibo Yuan, Hongling Liu, Jieke Du, Kaiquan Liu,Tengfei Wang*, Long Liu*. Biocatalytic production of 2,5-furandicarboxylic acid: recent advances and future perspectives. Applied Microbiology and Biotechnology, 2019, 104(2):527-543. IF:3.9, (5/6)通讯作者,三区(第一单位) 15.Tengfei Wang*, Dejun Chang, Di Huang*, Zetong Liu, Yukang Wu, Hongling Liu, Haibo Yuan, Yi Jiang. Application of surfactants in papermaking industry and future development trend of green surfactants. Applied Microbiology and Biotechnology, 2021, 105(20):7619-7634. IF: 3.9, (1/8)通讯作者,三区(第一单位) 16. Tianzhen Zhang, Lingpu Liu, Li Ye, Wenchao Li, Bo Xin, Yanyan Xie, Shiru Jia, Tengfei Wang*, Cheng Zhong*. The production of bacterial cellulose in Gluconacetobacter xylinus regulated by luxR overexpression of quorum sensing system. Applied Microbiology and Biotechnology, 2021, 105(20):7801-7811. IF: 3.9, (8/9)通讯作者,三区(第一单位) 17. Yi Jian, Xinyi Zhang, Haibo Yuan, Di Huang, Ruiming Wang, Hongling Liu*, Tengfei Wang*. Research progress and the biotechnological applications of multienzyme complex. Applied Microbiology and Biotechnology, 2021, 105(5):1759-1777. IF: 3.9, (7/7)通讯作者,三区(第一单位) 18. Ping Lin#, Haibo Yuan#, Jieke Du, Kaiquan Liu, Hongling Liu*, Tengfei Wang*. Progress in research and application development of surface display technology using Bacillus subtilis spores. Applied Microbiology and Biotechnology, 2020, 104(6):2319-2331. IF: 3.9, (6/6)通讯作者,三区(第一单位) 19. Hanyu Cong#, Haibo Yuan#, Zekun Tao, Hanlin Bao, Zheming Zhang, Yi Jiang, Di Huang, Hongling Liu, and Tengfei Wang*. Recent Advances in Catalytic Conversion of Biomass to2,5-Furandicarboxylic Acid. Catalysts, 2021, 11, 1113. IF: 3.8, (9/9)通讯作者,三区(第一单位) 20. Dengyue Sun, Jie Zhang, Chao Li, Tengfei Wang*, Hui-Min Qin*. Biochemical and structural characterization of a novel thermophilic and acidophilic beta-mannanase from Aspergillus calidoustus. Enzyme and Microbial Technology, 2021, 150:109891, IF:3.4, (4/5)通讯作者,三区(第一单位) 21. Wenhu Chen#, Qian Liu#, Meng Liu, Hongling Liu, Di Huang, Yi Jiang, Tengfei Wang*and Haibo Yuan*. Symbiosis Mechanism of Associated Bacteria on2-keto-L-gulonic Acid Production via Mixed Fermentation:A Review. Fermentation, 2023, 9, 1000. IF: 3.3, (7/8)通讯作者,三区(第一单位) 22. Hongling Liu, Xihui Wang, Shaojie Yang, Ruiming Wang,Tengfei Wang*. Saturation mutagenesis and self-inducible expression of trehalose synthase in Bacillus subtilis. Biotechnology Progress, 2019, 35(4):e2826. IF: 2.5, (5/5)通讯作者,三区(第一单位) 23. Liping Tan, Zhongyang Liu, Tongjun Liu* and Tengfei Wang*. Changes of the Structures and Properties of Corn Stover during Acid Sulfite Pretreatment for Improved Enzymatic Hydrolysis. BioResources, 2020, 15(1):1001-1013. IF:1.3, (4/4)通讯作者,三区(第一单位) 24. Zekun Tao#, Haibo Yuan#, Meng Liu, Qian Liu, Siyi Zhang, Hongling Liu, Yi Jiang,Di Huang, and Tengfei Wang*. Yeast Extract: Characteristics, Production,Applications and Future Perspectives. Journal of Microbiology and Biotechnology, 2023,33(2):151-166. IF:2.5, (9/9)通讯作者,四区(第一单位) 25. Xuejun Liu#, Yi Jiang#, Hongling Liu, Haibo Yuan, Di Huang and Tengfei Wang*. Research progress and biotechnological applications of feruloyl esterases. Biocatalysis and Biotransformation, 2023, 41(6):421-439. IF:1.4, (6/6)通讯作者,四区(第一单位)
发明专利: 1. 王腾飞,黄迪,常德军,武玉康,纪堂冰,刘静。一种预处理草浆原料脱硅的微生物菌剂及其在草浆原料除硅制浆中的应用。中国,ZL202210246052.1,2024-01-26,已授权 2. 王腾飞,黄迪,武玉康,孙晗,常德军,刘泽同。一种采用复合酶制剂预处理的秸秆制浆方法及其在制备纸吸管中的应用。中国,ZL202111546844.2,2023-02-07,已授权 3. 王腾飞,刘学军,蒋艺,刘洪玲,袁海波,黄迪。一种环糊精葡萄糖基转移酶突变体及应用。中国,ZL202210494671.2,2023-05-05,已授权 4. 王腾飞,黄迪,刘泽同,武玉康,孙晗,常德军。一种以复合秸秆为原料的可生物降解吸管及其制备方法。中国,ZL202210188776.5,2023-03-14,已授权 5. 王腾飞,陶泽坤,袁海波,刘洪玲,黄迪,蒋艺。一株在低糖环境下生产透明质酸的马链球菌兽疫亚种及其应用。中国,ZL202210418689.4,2023-05-09,已授权 6. 王腾飞,宋龙祥,刘洪玲,王冲,张慧敏,郑涵文。一种MTSase固定化酶和MTHase固定化酶的制备方法及其在海藻糖生产中的应用。中国,ZL202110204735.6,2022-12-23,已授权 7. 王腾飞,刘洪玲,王松。一种构建自组装表达双酶菌株的方法及应用。中国,ZL202010519285.5,2022-06-14,已授权 8. 王腾飞,林平,刘洪玲,马耀宏。一种在枯草芽孢杆菌芽孢表面展示葡萄糖氧化酶的方法与应用。中国,ZL201811490194.2,2022-05-24,已授权 9. 王腾飞,刘洪玲,杨少杰。一种在枯草芽孢杆菌的芽孢表面高效展示海藻糖合酶的方法。中国,ZL201811316783.9,2021-08-31,已授权 10. 王腾飞,刘洪玲,王瑞明,吕鑫。一种突变型海藻糖合酶的制备方法及应用。中国,ZL201711157105.8,2021-02-26,已授权 11. 王腾飞,宋龙祥,刘洪玲。一种同步分泌MTHase和MTSase重组枯草芽孢杆菌的构建及应用。中国,ZL202010106831.2,2021-12-21,已授权 12. 王腾飞,王希晖,刘洪玲,杨少杰。一种耦合发酵制备海藻糖的方法。中国,ZL201811310077.3,2021-08-31,已授权 13. 王腾飞,王瑞明,汪俊卿,王建彬,郭传庄。一种连续胞外酶生物法制备海藻糖的方法。中国,ZL201610323539.X,2021-01-22,已授权 14. 王腾飞,林平,刘洪玲。芽孢表面共展示葡萄糖氧化酶和过氧化氢酶的工程菌株及应用。中国,ZL201910753745.8,2021-04-13,已授权 15. 王腾飞,韩登兰,王瑞明,汪俊卿。一种芽孢表面稳定展示海藻糖合酶的工程菌及其构建方法。中国,ZL201611104170.X,2020-05-29,已授权 16. 王腾飞,刘洪玲,王希晖,王松。一种利用高效分泌表达的双酶融合酶耦合发酵生产海藻糖的方法。中国,ZL201811490196.1,2020-12-22,已授权 17. 王腾飞,王瑞明,刘强,隋松森。自诱导强化型海藻糖合酶合成工程菌的制备方法与应用。中国,ZL201610246849.6,2019-08-23,已授权 18. 王腾飞,王瑞明,王建彬,郭传庄。一种无臭赤藓糖醇的生产方法。中国,ZL201610244794.5,2019-05-03,已授权 19. 王腾飞,刘强,王瑞明。双启动子自诱导型重组枯草芽孢杆菌的构建方法与应用。中国,ZL201610839431.6,2019-10-15,已授权 20. 王腾飞,刘强,王瑞明。利用Pcry3Aa启动子构建高表达海藻糖合成酶工程菌的方法。中国,ZL2016103222822.6,2019-11-08,已授权 21. 王腾飞,孟武,王瑞明,王建彬,隋松森。一种四甲基吡嗪的提取纯化方法。中国,ZL201610242785.2,2018-07-31,已授权 22. 王腾飞,王瑞明,刘强。一种麦芽糖诱导型海藻糖合酶合成工程菌及其制备方法与应用。中国,ZL201510431599.9,2018-02-13,已授权 23. 王腾飞,王瑞明,王建彬,隋松森。一种固体多功能面膜组合物及制备面膜的方法。中国,ZL201610308040.1,2018-12-21,已授权 24. 王腾飞,王瑞明,李梦悦,陆奉勇。一种表面展示海藻糖合成酶、海藻糖水解酶的方法与应用。中国,ZL201510571619.2,2018-11-13,已授权 25. 王腾飞,王瑞明,刘强。一种淀粉诱导型重组枯草芽孢杆菌及其制备方法与应用。中国,ZL201510486544.8,2017-12-01,已授权 26. 王腾飞,王瑞明,李丕武。一种海藻糖的分离纯化方法。中国,ZL201310360328.X,2016-05-11,已授权
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